Journal: Frontiers in Immunology

Article Title: PhIP-driven prostate cancer involves key molecular regulators and immune microenvironment modulation

doi: 10.3389/fimmu.2026.1782240

Figure Lengend Snippet: PhIP exposure induces cytotoxicity in RWPE-1 cells and downregulates SLC14A1 expression in PC-3 cells. (A) Immunohistochemical staining of SLC14A1 in prostate cancer tissues and normal prostate tissues based on data from the HPA database. (B) Cell viability of RWPE-1 cells after treatment with increasing concentrations of PhIP for 48 h, as determined by CCK-8 assay. (C) Protein expression levels of SLC14A1 in PC-3 cells following PhIP treatment, as assessed by Western blot. (D) Relative mRNA expression levels of SLC14A1 in PC-3 cells after PhIP treatment, as determined by qRT-PCR (**p < 0.01, ***p < 0.001).

Article Snippet: The human prostatic epithelial cell line RWPE-1 and the human prostate cancer cell line PC-3 were obtained from the American Type Culture Collection (ATCC).

Techniques: Expressing, Immunohistochemical staining, Staining, CCK-8 Assay, Western Blot, Quantitative RT-PCR

Journal: Current Issues in Molecular Biology

Article Title: In Silico and In Vitro Investigation of Apoptosis-Mediated Antiproliferative Activity of Capsaicin and Alpha-Lipoic Acid Against Prostate Cancer Cells

doi: 10.3390/cimb48040376

Figure Lengend Snippet: Effect of different concentration of capsaicin on cell viability. Two different prostate cancer cell lines (PC-3 and DU-145) were treated with different concentrations of capsaicin for 24 and 48 h. The viability of cells was determined by MTT cell viability assay and expressed as percentage of the control. Data are shown as mean ± SD; * p < 0.05 compared with the control group.

Article Snippet: Prostate cancer cell lines PC-3 (CRL-1435) and DU-145 (HTB-81) were purchased from American Type Culture Collection (Manassas, VA, USA).

Techniques: Concentration Assay, Viability Assay, Control

Journal: Current Issues in Molecular Biology

Article Title: In Silico and In Vitro Investigation of Apoptosis-Mediated Antiproliferative Activity of Capsaicin and Alpha-Lipoic Acid Against Prostate Cancer Cells

doi: 10.3390/cimb48040376

Figure Lengend Snippet: Effect of different concentrations of ALA on cell viability. Two different prostate cancer cell lines (PC-3 and DU-145) were treated with different concentrations of ALA for 24 and 48 h. The viability of cells was determined by MTT cell viability assay and expressed as percentage of the control. Data are shown as mean ± SD; * p < 0.05 compared with the control group.

Article Snippet: Prostate cancer cell lines PC-3 (CRL-1435) and DU-145 (HTB-81) were purchased from American Type Culture Collection (Manassas, VA, USA).

Techniques: Viability Assay, Control

Journal: Current Issues in Molecular Biology

Article Title: In Silico and In Vitro Investigation of Apoptosis-Mediated Antiproliferative Activity of Capsaicin and Alpha-Lipoic Acid Against Prostate Cancer Cells

doi: 10.3390/cimb48040376

Figure Lengend Snippet: Effect of different concentrations of capsaicin and ALA combination on cell viability. Two different prostate cancer cell lines (PC-3 and DU-145) were treated with different concentrations of capsaicin and ALA for 24 and 48h. The viability of cells was determined by MTT cell viability assay and expressed as percentage of the control. Data are shown as mean ± SD; * < 0.05 compared with the control group; # < 0.05 compared with the lowest concentration of ALA (250 µM)-treated cells.

Article Snippet: Prostate cancer cell lines PC-3 (CRL-1435) and DU-145 (HTB-81) were purchased from American Type Culture Collection (Manassas, VA, USA).

Techniques: Viability Assay, Control, Concentration Assay

Journal: Current Issues in Molecular Biology

Article Title: In Silico and In Vitro Investigation of Apoptosis-Mediated Antiproliferative Activity of Capsaicin and Alpha-Lipoic Acid Against Prostate Cancer Cells

doi: 10.3390/cimb48040376

Figure Lengend Snippet: Effect of capsaicin, ALA and their combination on apoptosis-related protein expression in prostate cancer cells. Two different prostate cancer cell lines (PC-3 and DU-145) were treated with different concentration of capsaicin, ALA and their combination for 24 h. Survivin and bax protein expressions were determined using the Western blotting method ( B ). β-actin is used as a loading control. Quantitative analysis of survivin ( A ) and bax ( C ) protein. * p < 0.05 compared with the control group.

Article Snippet: Prostate cancer cell lines PC-3 (CRL-1435) and DU-145 (HTB-81) were purchased from American Type Culture Collection (Manassas, VA, USA).

Techniques: Expressing, Concentration Assay, Western Blot, Control

Journal: bioRxiv

Article Title: Dynamic optimization of extrachromosomal DNA copy number drives tumour evolution

doi: 10.64898/2026.03.20.713026

Figure Lengend Snippet: (a) Schematics depicting hypothesized ecDNA copy number distributions of all cells, G1 cells, and parent cells giving rise to daughter cells (left). Representative images from combined IF for Aurora B Kinase and Cyclin A and DNA FISH for MYC in COLO 320DM. Cyclin A was used as a marker to identify G1 interphase cells, whose copy number should theoretically be 1n before entering S-phase and undergoing genome doubling. Aurora B Kinase was used as an identification marker for recently divided daughter cells. Copy number of parents of daughter cells was estimated by taking the average of the daughter cells’ MYC DNA FISH foci areas. Copy numbers of parent cells were compared against copy numbers of G1 interphase cells to avoid high copy number bias arising from cells past S-phase and with replicated DNA content. Scale bars, 10 µm (right). (b) Histograms depicting proportion of G1 interphase and parent cells in each copy number quintile in COLO 320DM and PC3 DM.

Article Snippet: COLO 320DM and COLO 320HSR (colorectal cancer) and the parental PC3 (prostate cancer) cell lines were obtained from ATCC.

Techniques: Marker

Journal: bioRxiv

Article Title: IL12-engineered human PSMA-CAR T cells for the treatment of advanced prostate cancer

doi: 10.64898/2026.03.05.709907

Figure Lengend Snippet: Engineering hPSMA-CARs with mbIL12 rescues CAR T cell functionality a Schema of hPSMA-CAR variants with mbIL12 expression. b Flow cytometric analysis of ET260-1 CAR and ET260-1(58) CAR with or without mbIL12 as detected by extracellular Fc and intracellular IL-12. c Tumor cell killing of hPSMA-CARs at an E:T of 1:4 over 72 hrs. d Expression of 4-1BB over 72 hrs on hPSMA-CARs. e Expression of CD25 on hPSMA-CARs after 72 hrs. f Secretion of IFNγ of hPSMA-CARs with mbIL12 after 72 hrs as determined by ELISA. g Tumor cell killing of hPSMA-CARs with mbIL12 and J591 against PC-3 PIP at E:T of 1:10 over 5 days at varying concentrations of anti-IFNγR1 blocking antibody and isotype control. Data are presented as mean values ± SEM. P values indicate differences between ET260-1-dCH2(28tm)BBz and ET260-1-dCH2(28tm)BBz/mbIL12 and between ET260-1-dCH2(28tm)BBz/mbIL12 and J591-dCH2(4tm)BBz using a two-tailed Student’s t-test.

Article Snippet: Human metastatic prostate cancer cell line PC-3 (ATCC CRL-1435) was cultured in RPMI-1640 (Corning) containing 10% fetal bovine serum (FBS, Hyclone), and 1X antibiotic-antimycotic (AA, Gibco) (complete RPMI).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Blocking Assay, Control, Two Tailed Test

Journal: bioRxiv

Article Title: IL12-engineered human PSMA-CAR T cells for the treatment of advanced prostate cancer

doi: 10.64898/2026.03.05.709907

Figure Lengend Snippet: mbIL12-engineered hPSMA-CAR T cells demonstrate improved activity in a recursive tumor cell assay in vitro a Tumor cell killing of hPSMA-CARs over 8 days when co-cultured with PSMA-expressing cell lines at E:T of 1:20. b Fold expansion of hPSMA-CARs compared to J591-CAR after 8-day co-culture. c Production of IFNγ at 8 days as determined by ELISA. d Schema of rechallenge with PSMA-CARs co-cultured with PC-3 PSMA lo , PC-3 PSMA hi , or PC-3 PIP and rechallenged with tumor cells every 3 days. e PC-3 PSMA lo (left), PC-3 PSMA hi (center), or PC-3 PIP (right) cell counts were quantified by flow cytometry. b Fold expansion of T cells after each rechallenge were quantified by flow cytometry. c Production of IFNγ by T cells following each rechallenge as determined by ELISA. Data are presented as mean values ± SEM. P values indicate differences between ET260-1-dCH2(28tm)BBz and ET260-1-dCH2(28tm)BBz/mbIL12 and between ET260-1-dCH2(28tm)BBz/mbIL12 and J591-dCH2(4tm)BBz using a two-tailed Student’s t-test.

Article Snippet: Human metastatic prostate cancer cell line PC-3 (ATCC CRL-1435) was cultured in RPMI-1640 (Corning) containing 10% fetal bovine serum (FBS, Hyclone), and 1X antibiotic-antimycotic (AA, Gibco) (complete RPMI).

Techniques: Activity Assay, In Vitro, Cell Culture, Expressing, Co-Culture Assay, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Two Tailed Test